Mediaà[Type] ---------- Org. ß | [TransportMedia] | [OrdinaryMedia] | BloodAgar (BA) / ChocholateAgar (CA) | MacConkey (Lactose-fermentation) | [Selective/Enriched/Enrichment | Remarks |
Staphylococcus & |
| Golden Yellow Colonies | b-Hemolysis | Pink Colonies(+ve) | Black colonies on K-Tellurite media. Ludlam’s medium |
|
Streptococcus |
|
| a / b Hemolysis ( BA ) a-hemolysis (strepto.viridans) |
|
| Dick test for Scarlet-fever |
Streptococcus |
|
| a-Hemolysis Greenish discolouration around colonies |
|
| Carrom Coin appearance Quellung reaction (capsule) |
Neisseria |
|
| BA / CA used |
|
|
|
Neisseria | Stuart’s Transport media |
| BA / CA used Translucent Round colonies |
| Thayer-Martin Media (with Vancomycin, Nystatin, Colistin ) | 5-10%CO2 (Candle jar used)
|
Corynebacterium
|
|
| BA |
| Loeffler’s Serum slope K-Tellurite Blood agar (0.04%) (Macleod & Hoyle’s media) | Albert’s Stain for Metachromatic granules. Shick-test (diphteriasusceptibility) Elek’s gel ppt. Test & Guinea pig inoculation -for Toxigenicity / virulence |
Mycobacterium tuberculosis
|
|
|
|
| Lowenstein Jensen(L.J.) solid media. Dubos Liquid media Loeffler’s serum slope Petroff’s medium Dorset’s Egg | Auramine & Rhodamine staining for Fluorescent microscopy. NAP test +ve, Niacin test & Neutral Red test +ve |
Mycobacterium |
|
|
|
| Foot Pad of Mice ( 20oC) Nine Branded Armadillo. Human Foetal spinal Ganglion Cells | Do not grow in cell free culture |
Spirochaetes |
|
|
|
| No cultivation Only Dark-Ground Microscopy of Smears | Fontanas Method (smears) Levaditi Staining (tissue section) Nichol’s Strain (Rabbit Testes) |
Chlamydia trachomatis |
|
|
|
| Do not grow in cell-free media. Obligate intracellular org. | Inclusion bodies in Giemsa stain smears ELISA |
Salmonella typhi
|
| NA |
| Colourless Colonies. | -Selenite F & - Tetrathionate broth. -Deoxycholate citrate agar -Wilson&Blair medium(black colonies-s.typhi&s.paratyphi B.) Blood culture -Bile broth Castaneda’s double medium. | Widal testà (+ve at end of 1st wk.) NOTE:- BASU 1 2 3 4 wks. |
Vibrio cholerae
| VR- venkatraam-ramakrishnan medium Clary Blair medium | NA | (BA) (greening) ( Eltor) | Colourless colonies | 1) Alkaline peptone water-surface pellicle 2) BSA-alkaline Bile Salt Agar,ph8.0) 3) TCBS medium-Thiosulphate,citrate,bromthymaol bule & sucrose, 4) Monsur’s GTTA medium. | -String test -Hanging drop prep.-to see motility. -strains- Ogawa, Inaba, Hikojima Cholera red reaction-nitroso-indole forma’n |
Shigella |
|
|
| Colourless colonies Except—Sh.sonnei( late lactose ferm’n) | Deoxycholate citrate agar |
|
E. coli |
|
|
| Pink colonies |
|
|
Klebsiella |
|
|
| Pink colonies |
|
|
Proteus |
| NA |
| Colourless colonies | Tetrathionate Broth | Swarming inhibited by 6% Agar & Sodium Azide. PPA test +ve (deamination test) |
Pseudomonas |
| NA |
| Colourless |
| Pigment produced are—Pyocyanin,Fluroscin,Pyoverdin,Pyomelanin. |
Clostridium welchii | Stuart’s transport medium |
|
|
| Robertson’s cooked meat medium | -Subterminal spores -Lecithinase effect—Nagler reaction |
Cl.tetani | Stuart’s transport medium |
|
|
| Robertson’s cooked meat medium | -Central spores Drum stick app. |
Citrobcater, |
|
|
| Pink colonies |
|
|
Original Article Contributed By: Dr. K B Ravva