Mcqs Main | Emqs | Exams | Study | Journals | CPanel | Links | Visa | About Us | FAQS | Members | Book Mark
Netmedicos.com
Turning dreams of medicos into reality...!!!
Exams - AIPGMEE | AIIMS MD MS EE | DNB CET | UPSC CMS EE
Mcqs - AIPGME 2010 Mcqs | AIPGME 2009 Mcqs | AIPGME 2008 Mcqs | AIPGME 2007 Mcqs | AIPGME 2006 Mcqs | AIPGME 2005 Mcqs | AIPGME 2004 Mcqs
Specialities - Anatomy | Biochemistry | Physiology | Forensic Medicine & Toxicology | Pathology | Pharmacology | Parasitology | Microbiology | Community Medicine | Preventive & Social Medicine
Ophthalmology | Otorhinolaryngology | Paediatrics | Medicine | Surgery | Gynaecology | Obstetrics | Orthopaedics | Psychiatry | Radiodiagnosis | Radiotherapy | Anaesthesiology
Section Menu
Forums
Recent Topics
Post New Topic
Section Menu
Cultural Characteristics of Bacteria
Welcome, Guest
Login


Register | Lost Password
Main Menu
Home
AIIMS 2009 Nov Mcqs
Academies
Associations
Boards
Book Store
Career Options
Colleges
Conferences
Courses
Exams (New)
Foundations
Hospitals
India
MBBS
BDS
Mcqs (New Section)
Mcqs (Old Section)
MD/MS Exam Calendar
Medical Jokes
Mnemonics
Quotations
Research Awards
Research Fellowships
Societies
Study (New)
Study
Admin Updates
Mcq of The Day Menu
Anatomy
Biochemistry
Community Medicine
Gynaecology
Medicine
Microbiology
Obstetrics
Ophthalmology
Orthopaedics
Otorhinolaryngology
Paediatrics
Pathology
Pharmacology
Physiology
Psychiatry
Surgery
AIPGMEE Menu
AIPGMEE 2013
AIPGMEE 2012
AIPGMEE 2011
AIPGMEE 2010
AIPGMEE 2009
AIPGMEE 2008
AIPGMEE 2007
AIIMS MD MS EE Menu
AIIMS MD MS 2013 Nov
AIIMS MD MS 2013 May
AIIMS MD MS 2012 Nov
AIIMS MD MS 2012 May
AIIMS MD MS 2011 Nov
AIIMS MD MS 2011 May
AIIMS MD MS 2010 Nov
AIIMS MD MS 2010 May
AIIMS MD MS 2009 Nov
AIIMS MD MS 2009 May
AIIMS MD MS 2008 Nov
AIIMS MD MS 2008 May
AIIMS MD MS 2007 Nov
AIIMS MD MS 2007 May
WBPGMAT Menu
WBPGMAT 2007
UPSC CMS EE Menu
UPSC CMS 2013
UPSC CMS 2012
UPSC CMS 2011
UPSC CMS 2010
UPSC CMS 2009
UPSC CMS 2008
UPSC CMS 2007
Admissions
Ayush And Health Sci
Ayush & Health Scien
UPUMCWA UPPGMET 2011
COMEDK PGET 2011 NOT
Sri Siddhartha Unive
Recent Updates
UPSC-CMS
UPSC CMS 2009 Paper
UPSC CMS 2009
UPSC CMS 2009 Paper
UPSC CMS 2010 Paper
Mock Tests
Mock TestAIIMS MD/MS Mock Series

Happy B'days Today (5)
Happy Birthday Esveekay
Happy Birthday Roshan
Happy Birthday Yppins
Happy Birthday Zuel
Happy Birthday Mbatuegwu Chidi

Newest Member
Newest Member Ris247
Home >> Cultural Characteristics of Bacteria
Cultural Characteristics of Bacteria
Cultural Characteristics of Bacteria[By - Dr. K B Ravva]
 

Mediaà[Type] 

----------

Org. ß

[TransportMedia]

[OrdinaryMedia]
Nutrient
Agar (NA)
 

BloodAgar (BA) /

ChocholateAgar   (CA)

MacConkey
Medium

(Lactose-fermentation)

[Selective/Enriched/Enrichment
Media]

Remarks

Staphylococcus
aureus / pyogenes

&
Staphylococcus
albus / epidermidis

 

Golden Yellow Colonies

b-Hemolysis
(Complete)
(BA)

Pink Colonies(+ve)

Black colonies  on K-Tellurite media.

Ludlam’s medium
(salt-milk agar)

 

Streptococcus
pyogenes
(Group A)
 

 

 

a / b Hemolysis ( BA )

a-hemolysis (strepto.viridans)

 

 

Dick test  for Scarlet-fever

Streptococcus
pneumoniae

 

 

a-Hemolysis

Greenish discolouration around colonies

 

 

Carrom Coin appearance

Quellung reaction (capsule)

Neisseria
meningitidis (meningococci)   

 

 

BA / CA used
Translucent  Convex colonies

 

 

 

Neisseria
gonorrhoea (gonococci)

Stuart’s Transport media

 

BA / CA used Translucent  Round colonies

 

Thayer-Martin Media (with Vancomycin, Nystatin, Colistin )

5-10%CO2 (Candle jar used)

 

Corynebacterium
diptheriae

 

 

 

BA

 

Loeffler’s Serum slope

K-Tellurite Blood agar (0.04%)

(Macleod & Hoyle’s media)

Albert’s Stain for Metachromatic granules.

Shick-test (diphteriasusceptibility)

Elek’s gel ppt. Test & Guinea pig inoculation -for Toxigenicity / virulence

Mycobacterium tuberculosis

 

  

 

 

 

 

Lowenstein Jensen(L.J.) solid media.

Dubos Liquid media

Loeffler’s serum slope

Petroff’s medium

Dorset’s Egg
medium

Auramine & Rhodamine staining for Fluorescent microscopy.

NAP test +ve, Niacin test & Neutral Red test +ve

Mycobacterium
leprae
 

 

 

 

 

Foot Pad of Mice ( 20oC)

Nine Branded Armadillo.

Human Foetal spinal Ganglion Cells

Do not grow in cell free culture

Spirochaetes

 

 

 

 

No cultivation

Only Dark-Ground Microscopy of Smears

Fontanas Method   (smears)

Levaditi Staining (tissue section)

Nichol’s Strain (Rabbit Testes)

Chlamydia trachomatis

 

 

 

 

Do not grow in cell-free media.

Obligate intracellular org.

Inclusion bodies in Giemsa stain smears

ELISA

Salmonella typhi 

 

 

 

 

 

NA

 

Colourless

Colonies.

-Selenite F &

- Tetrathionate broth.

-Deoxycholate citrate agar

-Wilson&Blair medium(black colonies-s.typhi&s.paratyphi B.)

Blood culture
- Ist.wk.+ve (TSB -Trypsicase soy broth)

-Bile broth

Castaneda’s double medium.

Widal testà (+ve at end of 1st wk.)
‘O’titre > 1: 100 &
‘H’titer > 1:200 is significant.

NOTE:- BASU 1 2 3 4 wks.
B-blood Culture
A-Antibody / WIDAL estm’n
S-stool Culture
U-urine culture

Vibrio cholerae

 

 

 

VR- venkatraam-ramakrishnan medium

Clary Blair medium

NA

(BA)
(Partial Hemolysis)

(greening)

( Eltor)

Colourless colonies

1) Alkaline peptone water-surface pellicle

2) BSA-alkaline Bile Salt Agar,ph8.0)

3) TCBS medium-Thiosulphate,citrate,bromthymaol bule & sucrose,

4) Monsur’s GTTA medium.

-String test

-Hanging drop  prep.-to see motility.

-strains- Ogawa, Inaba, Hikojima

Cholera red reaction-nitroso-indole forma’n

Shigella

 

 

 

Colourless colonies

Except—Sh.sonnei( late lactose ferm’n)

Deoxycholate citrate agar

 

E. coli

 

 

 

Pink colonies

 

 

Klebsiella

 

 

 

Pink colonies

 

 

Proteus

 

NA
( Swarming Growth)

 

Colourless colonies

Tetrathionate Broth

Swarming inhibited by 6% Agar & Sodium Azide.

PPA test +ve (deamination test)

Pseudomonas

 

NA
(Greenish colonies Pyocanin)

 

Colourless

 

Pigment produced are—Pyocyanin,Fluroscin,Pyoverdin,Pyomelanin.

Clostridium welchii

Stuart’s transport medium
&
T
hioglycollate broth.

 

 

 

Robertson’s cooked meat medium
(this medium turns PINK)

-Subterminal spores

-Lecithinase effect—Nagler reaction

Cl.tetani

Stuart’s transport medium
&
T
hioglycollate broth.

 

 

 

Robertson’s cooked meat medium
( this medium turns BLACK)

-Central spores

Drum stick app.

Citrobcater,
Enterobacter,
Serratia

 

 

 

Pink colonies

 

 


Original Article Contributed By: Dr. K B Ravva

Google
Top Posters
Netmedicos (803)
kkrish (274)
superbdoc (273)
nanu (228)
juhidr (149)
sanju5 (138)
Angelina (130)
jemzcal (127)
ponnu (116)
Varunrag (72)
Netmedicos Experts
Angelina (124)
txguy (101)
Karuna Mujalda (50)
Netmedicos (43)
Sandhya (42)
Tanushree Pandi (21)
sudipto (20)
sanju5 (10)
Jiss Joseph Pan (10)
Smitha (10)
Top Links
Feedback