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Home >> Cultural Characteristics of Bacteria
Cultural Characteristics of Bacteria
Cultural Characteristics of Bacteria[By - Dr. K B Ravva]
 

Mediaà[Type] 

----------

Org. ß

[TransportMedia]

[OrdinaryMedia]
Nutrient
Agar (NA)
 

BloodAgar (BA) /

ChocholateAgar   (CA)

MacConkey
Medium

(Lactose-fermentation)

[Selective/Enriched/Enrichment
Media]

Remarks

Staphylococcus
aureus / pyogenes

&
Staphylococcus
albus / epidermidis

 

Golden Yellow Colonies

b-Hemolysis
(Complete)
(BA)

Pink Colonies(+ve)

Black colonies  on K-Tellurite media.

Ludlam’s medium
(salt-milk agar)

 

Streptococcus
pyogenes
(Group A)
 

 

 

a / b Hemolysis ( BA )

a-hemolysis (strepto.viridans)

 

 

Dick test  for Scarlet-fever

Streptococcus
pneumoniae

 

 

a-Hemolysis

Greenish discolouration around colonies

 

 

Carrom Coin appearance

Quellung reaction (capsule)

Neisseria
meningitidis (meningococci)   

 

 

BA / CA used
Translucent  Convex colonies

 

 

 

Neisseria
gonorrhoea (gonococci)

Stuart’s Transport media

 

BA / CA used Translucent  Round colonies

 

Thayer-Martin Media (with Vancomycin, Nystatin, Colistin )

5-10%CO2 (Candle jar used)

 

Corynebacterium
diptheriae

 

 

 

BA

 

Loeffler’s Serum slope

K-Tellurite Blood agar (0.04%)

(Macleod & Hoyle’s media)

Albert’s Stain for Metachromatic granules.

Shick-test (diphteriasusceptibility)

Elek’s gel ppt. Test & Guinea pig inoculation -for Toxigenicity / virulence

Mycobacterium tuberculosis

 

  

 

 

 

 

Lowenstein Jensen(L.J.) solid media.

Dubos Liquid media

Loeffler’s serum slope

Petroff’s medium

Dorset’s Egg
medium

Auramine & Rhodamine staining for Fluorescent microscopy.

NAP test +ve, Niacin test & Neutral Red test +ve

Mycobacterium
leprae
 

 

 

 

 

Foot Pad of Mice ( 20oC)

Nine Branded Armadillo.

Human Foetal spinal Ganglion Cells

Do not grow in cell free culture

Spirochaetes

 

 

 

 

No cultivation

Only Dark-Ground Microscopy of Smears

Fontanas Method   (smears)

Levaditi Staining (tissue section)

Nichol’s Strain (Rabbit Testes)

Chlamydia trachomatis

 

 

 

 

Do not grow in cell-free media.

Obligate intracellular org.

Inclusion bodies in Giemsa stain smears

ELISA

Salmonella typhi 

 

 

 

 

 

NA

 

Colourless

Colonies.

-Selenite F &

- Tetrathionate broth.

-Deoxycholate citrate agar

-Wilson&Blair medium(black colonies-s.typhi&s.paratyphi B.)

Blood culture
- Ist.wk.+ve (TSB -Trypsicase soy broth)

-Bile broth

Castaneda’s double medium.

Widal testà (+ve at end of 1st wk.)
‘O’titre > 1: 100 &
‘H’titer > 1:200 is significant.

NOTE:- BASU 1 2 3 4 wks.
B-blood Culture
A-Antibody / WIDAL estm’n
S-stool Culture
U-urine culture

Vibrio cholerae

 

 

 

VR- venkatraam-ramakrishnan medium

Clary Blair medium

NA

(BA)
(Partial Hemolysis)

(greening)

( Eltor)

Colourless colonies

1) Alkaline peptone water-surface pellicle

2) BSA-alkaline Bile Salt Agar,ph8.0)

3) TCBS medium-Thiosulphate,citrate,bromthymaol bule & sucrose,

4) Monsur’s GTTA medium.

-String test

-Hanging drop  prep.-to see motility.

-strains- Ogawa, Inaba, Hikojima

Cholera red reaction-nitroso-indole forma’n

Shigella

 

 

 

Colourless colonies

Except—Sh.sonnei( late lactose ferm’n)

Deoxycholate citrate agar

 

E. coli

 

 

 

Pink colonies

 

 

Klebsiella

 

 

 

Pink colonies

 

 

Proteus

 

NA
( Swarming Growth)

 

Colourless colonies

Tetrathionate Broth

Swarming inhibited by 6% Agar & Sodium Azide.

PPA test +ve (deamination test)

Pseudomonas

 

NA
(Greenish colonies Pyocanin)

 

Colourless

 

Pigment produced are—Pyocyanin,Fluroscin,Pyoverdin,Pyomelanin.

Clostridium welchii

Stuart’s transport medium
&
T
hioglycollate broth.

 

 

 

Robertson’s cooked meat medium
(this medium turns PINK)

-Subterminal spores

-Lecithinase effect—Nagler reaction

Cl.tetani

Stuart’s transport medium
&
T
hioglycollate broth.

 

 

 

Robertson’s cooked meat medium
( this medium turns BLACK)

-Central spores

Drum stick app.

Citrobcater,
Enterobacter,
Serratia

 

 

 

Pink colonies

 

 


Original Article Contributed By: Dr. K B Ravva

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